abstract：:The impact of the two adaptation-induced mutations in an improved xylose-fermenting Zymomonas mobilis strain was investigated. The chromosomal mutation at the xylose reductase gene was critical to xylose metabolism by reducing xylitol formation. Together with the plasmid-borne mutation impacting xylose isomerase activ...

journal_title：Biotechnology letters

authors： Agrawal M,Wang Y,Chen RR

更新日期：2012-10-01 00:00:00

abstract：:Fifty-two fungal isolates obtained from seawater were screened for anti-nematode activity. One isolate, an Acremonium sp., exhibited the highest activity against the pinewood nematode, Bursaphelenchus xylophilus. When it was grown at pH 7.0 and 24-26 °C, nematicidal metabolites were produced. These were heat-stable, w...

journal_title：Biotechnology letters

authors： Meng Q,Shi X,Meng F,Feng X,Sun J

更新日期：2012-10-01 00:00:00

abstract：:The protective effects of polymer additives, including a group of viscosity-enhancing polymer poly-γ-glutamic acid (γPGA; 10, 50, and 500 kDa) and surface-active polymer Pluronic F68, on Chinese hamster ovary cells against damage due to shear stress were investigated in shake-flask cultures. The level of protection wa...

journal_title：Biotechnology letters

authors： Chun BH,Lee YK,Chung N

更新日期：2012-10-01 00:00:00

abstract：:Washed cells (62 mg) of Kitasatospora sp. GF12 in 4 ml buffer (pH 7) catalyzed the regioselective hydroxylation of 60 mM 1,3-adamantanediol [1,3-ad(OH)(2)] to 30.9 mM 1,3,5-adamantanetriol [1,3,5-ad(OH)(3)] over 120 h at 24 °C. Glycerol at 400 mM was added to the reaction mixture to recycle the intracellular NADH/NADP...

journal_title：Biotechnology letters

authors： Mitsukura K,Yamanaka N,Yoshida T,Nagasawa T

更新日期：2012-09-01 00:00:00

abstract：:5-Aminolevulinic acid (ALA) is an intermediate in the biosynthesis of tetrapyrroles. Its current production is expensive. We have developed a low-cost medium for Propionibacterium acidipropionici to produce extracellular ALA. When grown at 35 °C on a medium containing 3 % (w/v) food-grade sodium lactate supplemented w...

journal_title：Biotechnology letters

authors： Sonhom R,Thepsithar C,Jongsareejit B

更新日期：2012-09-01 00:00:00

abstract：:Glycolipid biosurfactants produced by bacteria and yeasts provide significant opportunities to replace chemical surfactants with sustainable biologically produced alternatives in bulk commercial products such as laundry detergents and surface cleaners. Sophorolipids are already available in sufficient yield to make th...

journal_title：Biotechnology letters

authors： Marchant R,Banat IM

更新日期：2012-09-01 00:00:00

abstract：:Several alkyl-substituted methoxysilanes were evaluated as potential activity and stability enhancing agents for biomimetic silicification of Rhodosporidium toruloides D-amino acid oxidase (RtDAO). When methyl-substituted silanes along with tetramethoxysilane were used as silicic acid precursors for polyallylamine (PA...

journal_title：Biotechnology letters

authors： Kuan IC,Chuang CA,Lee SL,Yu CY

更新日期：2012-08-01 00:00:00

abstract：:Clostridium spp. produce n-butanol in the acetone/butanol/ethanol process. For sustainable industrial scale butanol production, a number of obstacles need to be addressed including choice of feedstock, the low product yield, toxicity to production strain, multiple-end products and downstream processing of alcohol mixt...

journal_title：Biotechnology letters

authors： Jurgens G,Survase S,Berezina O,Sklavounos E,Linnekoski J,Kurkijärvi A,Väkevä M,van Heiningen A,Granström T

更新日期：2012-08-01 00:00:00

abstract：:Eugenitin, a chromone derivative and a metabolite of the endophyte Mycoleptodiscus indicus, at 5 mM activated a recombinant GH11 endo-xylanase by 40 %. The in silico prediction of ligand-binding sites on the three-dimensional structure of the endo-xylanase revealed that eugenitin interacts mainly by a hydrogen bond wi...

journal_title：Biotechnology letters

authors： Andrioli WJ,Damásio AR,Silva TM,da Silva VB,Maller A,Nanayakkara NP,Silva CH,Polizeli ML,Bastos JK

更新日期：2012-08-01 00:00:00

abstract：:Avian myeloblastosis virus reverse transcriptase (AMV RT) is a heterodimer consisting of a 63 kDa α-subunit and a 95 kDa β subunit. Moloney murine leukaemia virus reverse transcriptase (MMLV RT) is a 75 kDa monomer. These two RTs are the most extensively used for conversion of RNA to DNA. We previously developed sever...

journal_title：Biotechnology letters

authors： Konishi A,Yasukawa K,Inouye K

更新日期：2012-07-01 00:00:00

abstract：:Since T cells express diverse sex steroid hormone receptors, they might be a good model to evaluate the effects of sex steroid hormones on immune modulation. Porcine testicular extract contains several sex steroid hormones and may be useful to study the effects of sex steroid hormones during T cell activation. We have...

journal_title：Biotechnology letters

authors： Lee HH,Lee SJ,Kim S,Jeong S,Na M,Lee DM,Cheon YP,Lee KH,Choi I,Chun T

更新日期：2012-07-01 00:00:00

abstract：:Electro-acupuncture (EA) at Fenglong acupoint (ST40) can lower the levels of serum cholesterol and triacylglycerols. To study the hepatic genes responsible for the cholesterol-lowering effect of EA, suppression subtractive hybridization combined with the switch mechanism at the 5'-end of RNA template cDNA synthesis an...

journal_title：Biotechnology letters

authors： Li L,Tan GH,Zhang YZ

更新日期：2012-07-01 00:00:00

abstract：:Fungal diseases damage crop plants and affect agricultural production. Transgenic plants have been produced by inserting antifungal genes to confer resistance against fungal pathogens. Genes of fungal cell wall-degrading enzymes, such as chitinase and glucanase, are frequently used to produce fungal-resistant transgen...

journal_title：Biotechnology letters

authors： Ceasar SA,Ignacimuthu S

更新日期：2012-06-01 00:00:00

abstract：:Coupling lactate fermentation with cell growth was investigated in shake-flask and bioreactor cultivation systems by increasing aeration to improve lactate productivity in Escherichia coli CICIM B0013-070 (ackA pta pps pflB dld poxB adhE frdA). In shake-flasks, cells reached 1 g dry wt/l then, cultivated at 100 rpm an...

journal_title：Biotechnology letters

authors： Zhou L,Tian KM,Niu DD,Shen W,Shi GY,Singh S,Wang ZX

更新日期：2012-06-01 00:00:00

abstract：:An extracellular esterase, EstK, was purified from the psychrotrophic bacterium Pseudomonas mandelii grown at 25°C. Prior to harvest, cells were treated with 0.2 M MgCl2 to precipitate lipopolysaccharides in the outer membranes, which otherwise form aggregates with the secreted enzymes. EstK was purified to homogeneit...

journal_title：Biotechnology letters

authors： Hong S,Lee C,Jang SH

更新日期：2012-06-01 00:00:00

abstract：:The actinorhizal plant seabuckthorn (Hippophae rhamnoides L., Elaeagnaceae) is a wind pollinated dioecious crop. To distinguish male genotypes from female genotypes early in the vegetative growth phase, we have developed robust PCR-based marker(s). DNA bulk samples from 20 male and 20 female plants each were screened ...

journal_title：Biotechnology letters

authors： Korekar G,Sharma RK,Kumar R,Meenu,Bisht NC,Srivastava RB,Ahuja PS,Stobdan T

更新日期：2012-05-01 00:00:00

abstract：:Aerobic granules for sulphide and ammonium removal were cultivated in a sequencing batch reactor, and the microbial community of the aerobic granules was investigated by denaturing gradient gel electrophoresis. The loading rate increased from 0.15 to 0.9 kg S2− m−3 d−1, and the removal efficiencies of sulphide, chemic...

journal_title：Biotechnology letters

authors： Su C,Zhu L,Zhang C,Qi X,Guo Y,Gao R

更新日期：2012-05-01 00:00:00

abstract：:Deer antlers are the only mammalian appendages capable of repeated rounds of regeneration. Every year, deer antlers are shed and regrown from blastema into large branched structures of cartilage and bone. Little is known about the genes involved in antler development particularly during the later stages of ossificatio...

journal_title：Biotechnology letters

authors： Yao B,Zhao Y,Zhang H,Zhang M,Liu M,Liu H,Li J

更新日期：2012-05-01 00:00:00

abstract：:Synthesis of long DNA fragments is often associated with mutations and requires multiple DNA manipulation steps. A novel DNA synthesis method, referred to as patch oligodeoxynucleotide synthesis (POS) to assembly long DNA fragments is presented here. This method involves connection of two types of oligodeoxynucleotide...

journal_title：Biotechnology letters

authors： Yang G,Wang S,Wei H,Ping J,Liu J,Xu L,Zhang W

更新日期：2012-04-01 00:00:00

abstract：:Constant medium feeding rate and intermittent fed-batch fermentation strategies were investigated aiming to increase the yields of γ-decalactone production by Yarrowia lipolytica, using methyl ricinoleate as substrate and ricinoleic acid source. The accumulation of another compound, 3-hydroxy-γ-decalactone, was also a...

journal_title：Biotechnology letters

authors： Gomes N,Teixeira JA,Belo I

更新日期：2012-04-01 00:00:00

abstract：:For most cultivated mammalian cells, glutamine is an essential medium component. However, glutamine consumption results in the production of ammonia, a cytotoxic byproduct. Here we investigated the effect of glutamine reduction on recombinant protein production and ammonia accumulation in transiently transfected CHO a...

journal_title：Biotechnology letters

authors： Rajendra Y,Kiseljak D,Baldi L,Hacker DL,Wurm FM

更新日期：2012-04-01 00:00:00

abstract：:The recombinant laccase from Thermus thermophilus was applied to the biobleaching of wheat straw pulp. The best bleaching effect was when the pulp was treated with 3 U laccase g(-1) dry pulp at 90°C, pH 4.5, 8% consistency for 1.5 h. Under these conditions, the pulp brightness was increased by 3.3% ISO, and the pulp k...

journal_title：Biotechnology letters

authors： Zheng Z,Li H,Li L,Shao W

更新日期：2012-03-01 00:00:00

abstract：:The basic reactions of the clostridial 1-butanol biosynthesis pathway can be regarded to be the inverted reactions of the fatty acid β-oxidation pathway. A pathway for the biosynthesis of fuels and chemicals was recently engineered by combining enzymes from both aerobic and anaerobic fatty acid β-oxidation as well as ...

journal_title：Biotechnology letters

authors： Gulevich AY,Skorokhodova AY,Sukhozhenko AV,Shakulov RS,Debabov VG

更新日期：2012-03-01 00:00:00

abstract：:Human DNA polymerase iota (Pol ι) is a Y-family DNA polymerase with unusual biochemical properties and not fully understood functions. Pol ι preferentially incorporates dGTP opposite template thymine. This property can be used to monitor Pol ι activity in the presence of other DNA polymerases, e.g. in cell extracts of...

journal_title：Biotechnology letters

authors： Makarova IV,Kazakov AA,Makarova AV,Khaidarova NV,Kozikova LV,Nenasheva VV,Gening LV,Tarantul VZ,Andreeva LE

更新日期：2012-02-01 00:00:00

abstract：:Two genes, MGFc (40) and MGFc (24), encoding different E peptides of mechano-growth factor (MGF), were obtained by a four-step PCR strategy and subcloned into pRSETC and pGEX-6p-1. Recombinant MGFc(40) protein (4 mg l(-1)) was expressed and purified by affinity chromatog...

journal_title：Biotechnology letters

authors： Song L,Zhu WS,Dong JL,Li ZY,Liu XH

更新日期：2012-02-01 00:00:00

abstract：:Biosurfactants could potentially outperform traditional surfactants in many applications whilst being more sustainable to source, manufacture, use and dispose of. However, currently available fermentation production methods are too inefficient to manufacture biosurfactants for these high volume markets. Foaming in an ...

journal_title：Biotechnology letters

authors： Winterburn JB,Martin PJ

更新日期：2012-02-01 00:00:00

abstract：:The effects of the sequential application of methyl jasmonate (MeJa), salicylic acid (SA) and yeast extract (YE) to Argemone mexicana cell cultures were compared to either the sole application of each elicitor, or to the three-partite mixture. The highest sanguinarine accumulation occurred using the sequential treatme...

journal_title：Biotechnology letters

authors： Trujillo-Villanueva K,Rubio-Piña J,Monforte-González M,Ramírez-Benítez E,Vázquez-Flota F

更新日期：2012-02-01 00:00:00

abstract：:A very high gravity (VHG) repeated-batch fermentation system using an industrial strain of Saccharomyces cerevisiae PE-2 (isolated from sugarcane-to-ethanol distillery in Brazil) and mimicking industrially relevant conditions (high inoculation rates and low O(2) availability) was successfully operated during fifteen c...

journal_title：Biotechnology letters

authors： Pereira FB,Gomes DG,Guimarães PM,Teixeira JA,Domingues L

更新日期：2012-01-01 00:00:00

abstract：:Therapies for high-grade gliomas (HHG) that have strong tendency of infiltration and resistance to chemotherapies are currently unavailable. Here, we report that lower-dose combination therapy of Buthus martensii Karsch (BmK) CT, a type of scorpion toxin peptide, and LiCl, clinically used as mo...

journal_title：Biotechnology letters

authors： Fu Y,Zheng S,Huang R,An N,Zheng Y,Zhang Z,Liang A

更新日期：2012-01-01 00:00:00

abstract：:The production of a low-temperature alkalophilic lipase from Acinetobacter johnsonii was improved using genome shuffling. The starting populations, obtained by UV irradiation and diethyl sulfate mutagenesis, were subjected to recursive protoplast fusion. The optimal conditions for protoplast formation and regeneration...

journal_title：Biotechnology letters

authors： Wang H,Zhang J,Wang X,Qi W,Dai Y

更新日期：2012-01-01 00:00:00

abstract：:Monascus aurantiacus produces high amounts of citrinin which is a mycotoxin with nephrotoxic activity. Six putative citrinin biosynthesis genes have been discovered in M. purpureus and at least 10 genes are responsible for its biosynthesis. However, the sequence of citrinin pathway gene cluster in M. aurantiacus has n...

journal_title：Biotechnology letters

authors： Li YP,Xu Y,Huang ZB

更新日期：2012-01-01 00:00:00

abstract：:The ability of Aspergillus strains to utilize thin stillage to produce malic acid was compared. The highest malic acid was produced by Aspergillus niger ATCC 9142 at 17 g l(-1). Biomass production from thin stillage was similar with all strains but ATCC 10577 was the highest at 19 g l(-1). The highest malic acid yield...

journal_title：Biotechnology letters

authors： West TP

更新日期：2011-12-01 00:00:00

abstract：:Human intestinal maltase (HMA) is an α-glucosidase that hydrolyses α-1,4-linkages from the non-reducing end of malto-oligosaccharides. HMA is an important target to discover of new drugs for the treatment of type 2 diabetes. In this study, 308,307 compounds were virtually screened with HMA using Autodock 3.0.5 in a WI...

journal_title：Biotechnology letters

authors： Nguyen TT,Ryu HJ,Lee SH,Hwang S,Cha J,Breton V,Kim D

更新日期：2011-11-01 00:00:00

abstract：:The open reading frame of the Δ(6)-desaturase gene was isolated from Mortierella alpina W15 and the gene was cloned into a pPIC3.5K vector. The vector was transformed into Pichia pastoris GS115 and expression was induced with methanol. The Δ(6)-desaturase expressed in P. pastoris GS115 catalyzed the conversion of lino...

journal_title：Biotechnology letters

authors： Liu J,Li D,Yin Y,Wang H,Li M,Yu L

更新日期：2011-10-01 00:00:00

abstract：:The lipase-catalyzed reaction is useful to obtain sugar esters with chemically defined structures and will contribute to the synthesis of sugar-based compounds by a chemo-enzymatic pathway. The synthesis of sugar esters in nonaqueous media has been attempted for a quarter century. To facilitate the reactions, they hav...

journal_title：Biotechnology letters

authors： Kobayashi T

更新日期：2011-10-01 00:00:00

abstract：:Cell-based therapeutics are promising routes for the regeneration of damaged cells and organs. The recovery of cells cultured in vitro for such applications requires the use of proteolytic enzymes which deteriorate its property by disruption of cell-cell and cell-matrix interactions. Intact cell sheets can be retrieve...

journal_title：Biotechnology letters

authors： Mukundan LM,Nirmal R,Thomas LV,Sajeev US,Nair PD

更新日期：2011-10-01 00:00:00

abstract：:Error-prone PCR was used to create more thermoactive and/or thermostable variants of thermoalkalophilic lipases. A variant of the α6 helix (lid domain), with an 189E to V substitution at residue 189, lost its thermostability but exhibited higher activity than that of its wild-type predecessor (r03Lip). Site-saturation...

journal_title：Biotechnology letters

authors： Shih TW,Pan TM

更新日期：2011-09-01 00:00:00

abstract：:Tandem affinity purification (TAP) is a methodology for the isolation of protein complexes from endogenous sources. It involves incorporation of a dual-affinity tag into the protein of interest and introduction of the construct into desired cell lines or organisms. Using the two affinity handles, the protein complex a...

journal_title：Biotechnology letters

authors： Li Y

更新日期：2011-08-01 00:00:00

abstract：:Continuous production of fructooligosaccharides (FOS) by Aureobasidium pullulans immobilized on calcium alginate beads with a packed bed was investigated at a plant scale reactor. Optimum conditions were with 770 g sucrose/l, being fed at 200 l/h at 50°C which gave a productivity of 180 g FOS/l h. Initial activity was...

journal_title：Biotechnology letters

authors： Jung KH,Bang SH,Oh TK,Park HJ

更新日期：2011-08-01 00:00:00

abstract：:An endoxylanase gene, xynA, was cloned from Bacillus pumilus ARA and expressed in Escherichia coli. The open reading frame of the xynA gene was 687 bp encoding a signal peptide and a mature xylanase with a molecular mass of 23 kDa. The enzyme was categorized as a glycosyl hydrolase family 11 member based on the sequen...

journal_title：Biotechnology letters

authors： Qu W,Shao W

更新日期：2011-07-01 00:00:00